Brexit Party MEPs turn backs on EU anthem as protests mar first session of new parliament
Leader Nigel Farage and newly elected candidates including Ann Widdecombe and Annunziata Rees-Mogg performed the protest as representatives gathered for the first session since May's European elections.
Ukip's MEPs - then led by Mr Farage - performed the same political stand at the start of the session in 2014.
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Hide AdPaul Nuttall, the party's deputy leader at the time, said it was intended to send a message that they did not "recognise or respect the EU flag or anthem".
Others in the parliament refused to stand at all as the EU's anthem - composed by Ludwig van Beethoven in 1824 - was played by a jazz ensemble.
Reflecting the political divisions being felt over Brexit in the UK, Liberal Democrat MEPs took their seats wearing yellow "bollocks to Brexit" T-shirts.
The Brexit Party and Lib Dems - each with opposing views on leaving the EU - came first and second respectively in the EU polls.
Protests were also held against the decision to deny a seat to Catalan separatist Carles Puigdemont and in defence of a German ship captain being held in Italy in a row over migrant rights.
Tuesday marks the opening of the new five-year session of the parliament, though the length of the UK's involvement remains in doubt.
UK MEPs may sit in the parliament until the country formally leaves the EU.
A deadline of October 31 has currently been set for the UK to leave, though this could be extended if a deal is not found by then.